The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Applications
FC, ICFC - Quality tested
Application Notes
Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen section2.
Application References
1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP WB) 2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP WB IHC) 3. Olver JA, et al. 2012. Am J Physiol Renal Physiol. 302:1362. PubMed.
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Description
Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.