The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application References
Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744
Description
CD47, also known as Integrin-Associated Protein (IAP) ,is a membrane protein of about 50 kd with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region.It is widely expressed on many cell types and often associated with beta 3 integrins.The significance of this molecules is recently drawing increasing attention.It has been reported that CD47 functions as a self marker.Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages.By binding to SIRPalpha,CD47 controls hemostatic innate immune functions,such as phagocytosis and cell trafficking.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.