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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
TaKaRa Ex Taq Hot Start Version
- 供应商:
南京赛泓瑞
- 规格:
250U
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文献和实验SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
an automatic “hot start” in PCR and increasing sensitivity, specificity, and yield. Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3’ to 5’ exonuclease activity. Mixture of this enzyme with Taq DNA polymerase results in a six-fold
在丁香园见过很多大佬分享载体构建经验,所以我这里写的T载体构建只能算是入门级,给新手提供一点思路,有什么不足之处还希望大家多多指正。 我克隆的这个片段大小是1.8kb,用的是TaKaRa的LA-Taq酶做的PCR。 反应体系:5*buffer 5 ul MgCl2 5 ul dNTPs 8 ul Primer 1 1 ul Primer 2 1 ul LA-Taq 0.5 ul cDNA 1 ul (1ug左右基因组DNA) ddH2O up to 50 ul
Single tube confirmation PCR protocol
until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive
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