The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications
ICFC - Quality tested
Application Notes
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. No. 503702/503704) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization1,7: The LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503812) is recommended for neutralization. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2, in vivo capture7, and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431001 to 431006) are specially developed and recommended.
Application References
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. 2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. 3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. 4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. 5. Mo X, et al. 1995. J. Virol. 69:1288. 6. Karulin A, et al. 2000. J. Immunol. 164:1862. 7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28. 8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed 9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382. 10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
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Description
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.