Clone |
MP4-25D2 |
Isotype |
Rat IgG1, κ |
Reactivity |
Human, Cross-Reactivity: Swine (Pig, Porcine), Rhesus |
Immunogen |
CHO-expressed, recombinant human IL-4 |
Formulation |
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation |
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
Concentration |
0.5mg/ml |
Recommended Usage |
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-4 protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. |
Applications |
ELISA Detection, ELISPOT Detection, ICFC |
Application Notes |
ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody. Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4. |
Application References |
1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Dectection, Neut) 2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut) 3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Dectection, Neut) 4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Dectection) 5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Dectection) 6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC) 7. Raqib R, et al. 1995. Infect. Immun. 63:289. 8. Andersson J, et al. 1994. Immunology 83:16. 9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed 10. Kubota M, et al. 1997. J. Immunol. 158:5321. 11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed " |
Description |
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. |
Storage |
The antibody solution should be stored undiluted at 4°C. Do not freeze. |