E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µl per 5 ml antibody dilution buffer for each mini-gel (0.5 µg/ml). Additionally, each lot of this antibody is quality control tested by immunofluorescence microscopy. Immunofluorescence microscopy, suggested working dilution(s): Use 5 µl per 1 ml antibody dilution buffer (2.5 µg/ml). It is recommended that the reagent be titrated for optimal performance for each application.
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Applications
WB, IF, ICFC - Quality tested IHC - Validated
Description
The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.