The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
0.2
Concentration Units
mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining and flow cytometric analysis.
Application References
1. Tertilt C, et al. 2009. Infect Immun. 77:3044. (ELISA)PubMed
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Description
The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells. The RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.