The antibody was purified by affinity chromatography, and conjugated with PE/Cy5.5 under optimal conditions. The solution is free of unconjugated PE/Cy5.5 and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
"
Applications
FC - Quality tested
Application References
1. Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press.
Description
CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.