The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Applications
FC - Quality tested
Application Notes
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References
1. Pulford K, et al. 1992. Immunology 75:588. (IP) 2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320. 3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. 4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Description
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, Hemoglobin-Haptoglobin Complex Receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP) resulting in soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.