The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
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Application References
1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA) 2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA) 3. Velu V, et al. 2007. J. Virol. 81:5819. 4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed 5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed 6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA) 7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed 8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed 9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed 10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed 11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed 12. Salisch NC, et al. 2010. J. Immunol. 184:476.
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Description
The program death 1 (PD-1) receptor CD279 is a 55 kD member of the immunoglobulin superfamily. CD279/PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cells and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279/PD-1 and are members of the B7 gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279:PD-ligands results in inhibition of T cell proliferation and cytokine secretion.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.