The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested WB, ELISA - Reported in the literature
Application Notes
Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.
Application References
1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed 2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed 3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
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Description
β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
Storage
The antibody solution should be stored undiluted at 4°C.