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PE anti-human CD119 (IFN-γ R α chain)参数介绍>>>>
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: Western blotting3,4, immunoprecipitation3,4, immunofluorescence microscopy6. GIR-94 antibody does not inhibit IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308704) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308706) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References
1. Sheehan K, et al. 1988. J. Immunol. 140:4231. 2. Jouanguy E, et al. 2000. J. Clin. Invest. 105:1429. 3. Kaplan D, et al. 1996. J. Biol. Chem. 271:9. (WB IP) 4. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (WB IP) 5. Choi JC, et al. 2007. J. Immunol. 178:1598. 6. Humlicek AL, et al. 2007. J. Immunol. 178:6395. (IF)
Description
CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.