The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Description
CD104 is a 205 kD type I transmembrane glycoprotein, known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) forms α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.