Clone |
MP4-25D2 |
Isotype |
Rat IgG1, κ |
Reactivity |
Human, Cross-Reactivity: Swine (Pig, Porcine), Rhesus |
Immunogen |
CHO-expressed, recombinant human IL-4 |
Formulation |
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
Preparation |
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody. |
Recommended Usage |
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. |
Applications |
ICFC - Quality tested |
Application Notes |
ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody. Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4. |
Application References |
1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Dectection, Neut) 2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut) 3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Dectection, Neut) 4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Dectection) 5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Dectection) 6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC) 7. Raqib R, et al. 1995. Infect. Immun. 63:289. 8. Andersson J, et al. 1994. Immunology 83:16. 9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed 10. Kubota M, et al. 1997. J. Immunol. 158:5321. 11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed " |
Description |
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. |
Storage |
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |