The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration
test sizes: lot-specific; µg sizes: 0.2 mg/ml
Recommended Usage
Each lot of this mouse IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
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Applications
FC, ICFC - Quality tested
Application Notes
The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400224) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400264) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References
1. Luckashenak NA, et al. 2006. J. Immunol. 177:5177. 2. Burman AC, et al. 2007. Blood 110:1064. 3. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200. 4. Podolin PL, et al. 2008. J. Immunol. 180:7989. PubMed
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Description
The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.