The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.
Application References
1. Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC) 2. Drannik AG, et al. 2012.J. Virol. 86:4599. PubMed.
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Description
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.