The antibody was purified by affinity chromatography, and conjugated with PE/Cy7under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
FC - Quality tested
Application Notes
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Application References
1. Kan EA, et al. 1983. J. Immunol. 131:536. 2. Wood GS, et al. 1985. Am. J. Pathol. 120:371. 3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB) 4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC) 5. Carbone A, et al. 1999. Blood 93:2319. (IHC) 6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF) 7. Rutjens E, et al. 2007. J. Immunol. 178:1702. 8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Description
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.