The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5, in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 115808).
Application References
1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP) 2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP) 3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC) 4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block) 5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP) 6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block) 7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed 8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed 9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
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Description
CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.