The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
背景资料
This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]
Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]
Tested and found negative for ectromelia virus (mousepox).