DESCRIPTION CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal
muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable. Our Creatine Kinase Assay Kit is based on enzyme coupled reactions. The produced NADPH is measured at 340 nm and is proportionate to the CK activity in the sample.
APPLICATIONS Direct Assays: CK in serum, plasma and other biological samples. Pharmacology: effects of drugs on CK activity.
KEY FEATURES Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
STORAGE CONDITIONS This kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of at least 6 months.