肌酸激酶测试盒 | EnzyChrom™ Creatine

产品名称 ：
肌酸激酶测试盒 | EnzyChrom™ Creatine Kinase Assay Kit

分类名称 :
酶活性测定,血液尿液化学

产品介绍 :
测定方法 比色法（340nm）定量测定肌酸激酶活性。

产品说明 肌酸激酶（CK），也被称作肌氨酸磷酸激酶（CPK）是一种主要在骨骼肌﹑平滑肌以及大脑组织中表达的酶。它主要由两个亚基组成，即脑型亚基（B）和肌肉 型亚基（M）。且有三种同功酶：CK-MM﹑CK-BB和CK-MB。肌酸激酶能够催化肌氨酸转化为磷酸肌酸，并伴随有ATP的消耗和ADP的产生，同时 也可催化其逆反应。肌酸激酶通常能在急性胸痛和肾衰竭的病人体内检测到。体内高浓度的肌酸激酶常意味着肌肉损伤，且常见于受伤﹑横纹肌溶解﹑心肌梗塞﹑肌 炎﹑心肌炎﹑恶性高烧﹑抗精神病药恶性综合征等疾病。体内低浓度的肌酸激酶则意味着酒精性肝病和风湿性关节炎。简单﹑直接﹑高度自动化的肌酸激酶活性检测 手段是十分理想的。本公司产品检测基于酶联反应生成NADPH，在波长340nm下检测，其颜色深浅程度与样品中肌酸激酶活性成正比。

产品特点 测量敏感、准确：采用96孔板测试，肌酸激酶活性检测范围在5-00U/L之间。 操作简便：在室温或37℃环境下，整个过程只需加入工作试剂，并在10分钟和40分钟时读取吸光度即可。高通量：通过96孔板上的高通量自动检测手段，每 天可以测试上千个样本。

产品用途 1. 直接检测血清、血浆、尿液和生物制剂中肌酸激酶的活性; 2. 用于药品和药理学研究，检测药物对肌酸激酶活性的影响。

储藏条件 用冰运输，试剂和标准品在-20℃下保存。

DESCRIPTION CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal
muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable. Our Creatine Kinase Assay Kit is based on enzyme coupled reactions. The produced NADPH is measured at 340 nm and is proportionate to the CK activity in the sample.

APPLICATIONS Direct Assays: CK in serum, plasma and other biological samples. Pharmacology: effects of drugs on CK activity.

KEY FEATURES Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

STORAGE CONDITIONS This kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of at least 6 months.

其他 :
编号：ECPK-048, ECPK-100
测试：48T, 100T

公司简介 ：
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒，广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等)，安全可靠，适合各类仪器(分光光度仪、微孔读数仪等）。产品具有快速、简单、高通量（可用于HTS 药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便，用户不需花额外的时间来作调适测试。
博世生物产品主要包括：1、血液尿液化学；2、酶活性测定；3、能量代谢；4、氧化应激；5、高通量HTS试剂；6、阴阳离子检测。