脱氧核糖核酸(DNA)测试盒 | QuantiFluo™ D

产品名称：
脱氧核糖核酸(DNA)测试盒 | QuantiFluo™ DNA Assay Kit

分类名称 :
代谢产物分析

产品介绍 :
测定方法 荧光法（340nm/450nm）定量测定DNA的浓度。

Description DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. our QuantiFluo^TM DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at 450nm (lexc = 350nm), is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Key features Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 – 2,000 ng/mL) calf thymus DNA in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.

Applications: Direct Assays: plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.

Storage conditions. The kit is shipped at room temperature. Store the DNA standard at -20°C and the Reagent at 2-8°C. Shelf life of at least 12 months (see expiry dates on labels).

其他 :
编号：QFDN-048，QFDN-250
测试：48T,250T

公司简介：
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒，广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等)，安全可靠，适合各类仪器(分光光度仪、微孔读数仪等）。产品具有快速、简单、高通量（可用于HTS 药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便，用户不需花额外的时间来作调适测试。
博世生物产品主要包括：1、血液尿液化学；2、酶活性测定；3、能量代谢；4、氧化应激；5、高通量HTS试剂；6、阴阳离子检测。