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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品批号
- 英文名：
20S Proteasome Assay Kit (SDS Activation Format)
- 库存：
大量
- 供应商：
嵘崴达
- CAS号：
见产品说明
- 保存条件：
见产品说明
- 规格：
1KT
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主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右到货。
R&D试剂，周三定货，2-3周到货。
SIGMA试剂，周一，五定货，3周左右到货。
Millipore试剂，每周三定货，2-3周到货。
Invitrogen试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，周三定货，2-3周到货。
eBioscience试剂，部分常用现货，周三定货，2-3周到货。
STANT CRUZ试剂，周三定货，2-3周到货。
Biolegend试剂，周三定货，2-3周到货。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
USB试剂，常备现货，周五定货，4周到货。
Amresco试剂，常备现货，3-4周到货。
罗氏原料，请来电来邮咨询。
Thermo试剂，pierce, Hyclone部分常用现货。
主要经营的耗材品牌：
1. BIOHIT，单道移液半支消毒长期促销，350/支。
2. BD Fa2. BD Falcon耗材，常用常备现货。
| K-900 | 20S Proteasome Assay Kit (SDS Activation Format) (1 KT) | 1 KT | 3,580.00 |
| K-910 | S-100 Fraction Degradation Kit, HeLa Cells (1 KT) | 1 KT | 3,510.00 |
| K-915 | S-100 Fraction Conjugation Kit, HeLa Cells (1 KT) | 1 KT | 4,230.00 |
| K-920 | Fraction II Degradation Kit, HeLa Cells (1 KT) | 1 KT | 3,500.00 |
| K-925 | Fraction II Degradation Kit, Rabbit Reticulocyte (1 KT) | 1 KT | 3,500.00 |
| K-930 | Fraction II Conjugation Kit, HeLa Cells (1 KT) | 1 KT | 4,230.00 |
| K-935 | Fraction II Conjugation Kit, Rabbit Reticulocyte (1 KT) | 1 KT | 4,230.00 |
| K-960 | Ubiquitin Conjugation Kit (1 KT) | 1 KT | 5,010.00 |
| K-980 | Recombinant Human UbcH (E2) Enzyme Set (1 KT) | 1 KT | 3,970.00 |
| K-985 | Recombinant Human UbcH (E2) Enzyme Set, Dominant Negative (1 KT) | 1 KT | 2,150.00 |
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文献和实验Assays for Proteasome Assembly and Maturation
The 20S proteasome is a complex multisubunit protease that is present in all phylae of life. Eukaryotic 26S proteasomes, which are composed of 20S proteasomes and 19S activator complexes, mediate the degradation of ubiquitylated proteins
were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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