Culture Method: Complete Growth Medium: RPMI-1640 +10% FBS+双抗 Culture Conditions: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1×105 viable cells/mL. Do not allow cell concentration to exceed 1×106
cells/mL. Interval: Maintain cell density between 1×105 and 1×106 viable cells/mL. Medium Renewal: Every 2 to 3 days Cryopreservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase