Description Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level.our QuantiChromTM Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to only 40 min.
Key features Sensitive and accurate. Detection range 0.1 - 50 mM in 96-well plate.Rapid and reliable. Using optimized Cd/Cu reagent, the time required for reduction of NO3- to NO2- is 15 min at >98.5% conversion rate. Simple and high-throughput. The procedure involves mixing sample with two reagents, incubation for 5 min and reading the optical density. Can be readily automated to measure thousands of samples per day.
Applications: Direct Assays: NO in plasma, serum, urine, tissue/cells and foods.Drug Discovery/Pharmacology: effects of drugs on NO metabolism.
Storage conditions. The kit is shipped at room temperature. Store Reagent A, B and Nitrite standard at 4°C. All other components can be stored at room temp. Shelf life: at least 6 months (see expiry dates on labels).