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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-Human CD11b (activation epitope) FITC
- 规格：
25tests
human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes
after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1
on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in
adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain
on the α chain of Mac-1 very close to the ligand binding site in a region that is
widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and
inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion
Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing
of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1
in these unstimulated samples. However, higher levels of Mac-1 expression are observed in
activated samples when compared to unstimulated cells.
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文献和实验Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
Activation and Expansion of Human Treg Cells
to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer
Collection, Separation, and Activation of Human T Lymphocytes
(3 ). In particular, activation of antigen-specific CD4+ T cells of the Th2 subset in the lungs, resulting in interleukin (IL)-5 secretion, plays a major role in allergic inflammation of the airways (4 ). T-cell activation requires two signals, one provided
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