人类乳头状瘤病毒18 E6单克隆抗体

英文名称 HPV18 E6 protein
中文名称 人类乳头状瘤病毒18 E6单克隆抗体
别 名 E6; Human Papilloma Virus; Human papillomavirus type 18 E6; Human papillomavirus type 18 (E6); Human papillomavirus type 18 E6; Human papillomavirus type 18 E6 protein; Protein E6; HPV18 E6 protein; VE6_HPV18.
人类乳头状瘤病毒18 E6单克隆抗体
说 明 书 0.2ml
研究领域 肿瘤 免疫学 细菌及病毒
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 2F6
交叉反应 HPV18
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
人类乳头状瘤病毒18 E6单克隆抗体
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 11kDa
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from HPV18 E6 protein
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 产 商 BIOSS
PubMed PubMed
产品介绍 background:
Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Function:
Transcriptional transactivator. Binds double stranded DNA (By similarity). Has transforming activity. Inactivates, with E6-AP ubiquitin-protein ligase, the human p53/TP53 tumor suppressor protein by targeting it to degradation. Binds and targets human MUPP1/MPDZ protein to degradation. Those two functions presumably contribute to transforming activity. Interaction with human FBLN1 protein also seems to be linked to cell transformation.
人类乳头状瘤病毒18 E6单克隆抗体