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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate
- 规格：
25ul
描述
This mouse monoclonal antibody to the human CD3 antigen is conjugated to a Qdot® nanocrystal that has a peak emission wavelength of 655 nm.• Validated for use with Flow Cytometry applications
• Qdot® nanocrystals are photostable and do not need to be protected from light.
• Qdot® nanocrystals exhibit intrinsic brightness that can often be greater than other fluorophores.
Every Lot is Tested by Flow Cytometry
Each lot has been tested by flow cytometry using human peripheral blood leukocytes (PBLs). This testing was performed using 1 µL of antibody per 1 × 10e6 cells in a 100 µL staining volume (10 nM final concentration of Qdot® nanocrystals). Qdot® nanocrystal concentration is assigned based on optical density.
Get Brightness and Stability with Qdot® Nanocrystals
Qdot® nanocrystals are nanometer-scale atom clusters of semiconductor material which exhibit narrow and symmetrical emission bandwidths with very long Stokes shifts. The nanocrystals can be excited with any
CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate 引文和参考文献 (11)
- 作者
- Introini A, Vanpouille C, Lisco A, Grivel JC, Margolis L
- 杂志
- PLoS Pathog 2013; (9):2 e1003148-e1003148
- ID:
- PN135331
-
Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes
- 作者
- Telford WG
- 杂志
- Others (2004) 61A:9-17
- ID:
- PN129979
- 作者
- Perfetto SP, Chattopadhyay PK, Roederer M
- 杂志
- Nat Rev Immunol (2004) 4:648-655
- ID:
- PN56854
- 作者
- Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C
- 杂志
- Nature (321):431-434
- ID:
- PN130161
- 作者
- van den Elsen P, Shepley BA, Borst J, Coligan JE, Markham AF, Orkin S, Terhorst C
- 杂志
- Nature (312):413-418
- ID:
- PN130220
详细说明
常用规格
| Reactivity: | Human |
| Validated Application: | Flow Cytometry |
| Host: | Mouse |
| SubClass (Isotype): | IgG2a |
| Clonality: | Monoclonal |
| Clone ID: | S4.1 |
| Target Gene: | CD3 |
| Gene ID: | 915 |
| Label or Dye: | Qdot® 655 |
| Excitation⁄Emission (nm): | 405(488)⁄655 |
| Number of Reactions: | 25 Tests |
| Product Size: | 25 µL |
| Shipping Condition: | Wet Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
内容和储存
This product is supplied as 25uL of a 1 µM solution (in 50 mM borate, 1 M betaine, pH 8.3 that contains 0.05% sodium azide). Store at 2–8°C. Do not freeze.
荧光光谱
Qdot 655
Fluorescence Ex/Em spectra of Qdot® 655 in pH 7.2 buffer.
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
8 for that tube. 10) Resuspend the bead pellet by adding 0.5 mL of buffer to each sample tube. 11) Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12) Add
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