CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate

CD3 Mouse Anti-Human mAb (clon

e S4.1), Qdot® 655 conjugate
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  • ¥1892
  • Molecular Probes
  • Q10484
  • 2025年07月08日
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    • 详细信息
    • 技术资料
    • 抗体英文名

      CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate

    • 规格

      25ul

    CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate

    描述

    This mouse monoclonal antibody to the human CD3 antigen is conjugated to a Qdot® nanocrystal that has a peak emission wavelength of 655 nm.

    • Validated for use with Flow Cytometry applications
    • Qdot® nanocrystals are photostable and do not need to be protected from light.
    • Qdot® nanocrystals exhibit intrinsic brightness that can often be greater than other fluorophores.

    Every Lot is Tested by Flow Cytometry
    Each lot has been tested by flow cytometry using human peripheral blood leukocytes (PBLs). This testing was performed using 1 µL of antibody per 1 × 10e6 cells in a 100 µL staining volume (10 nM final concentration of Qdot® nanocrystals). Qdot® nanocrystal concentration is assigned based on optical density.

    Get Brightness and Stability with Qdot® Nanocrystals
    Qdot® nanocrystals are nanometer-scale atom clusters of semiconductor material which exhibit narrow and symmetrical emission bandwidths with very long Stokes shifts. The nanocrystals can be excited with any wavelength below their emission maximum, but are best excited by UV or violet (405 nm) light. Qdot® nanocrystals demonstrate an intrinsic brightness and photostability that can be many times greater than observed with other classes of fluorophores. These advantages make Qdot® nanocrystals powerful tools for antibody staining. The cytometer must have an appropriate emission filter for the Qdot® nanocrystal being used.

    Easy Staining
    Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA).

    Specific for CD3
    According to the literature this antibody recognizes the CD3 antigen. CD3 is comprised of 5 individual proteins that are responsible for T-cell receptor expression and signaling. CD3 is expressed on thymocytes, T-cells and some NK cells.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    CD3 Mouse Anti-Human mAb (clone S4.1), Qdot® 655 conjugate 引文和参考文献 (11)

    作者
    Introini A, Vanpouille C, Lisco A, Grivel JC, Margolis L
    杂志
    PLoS Pathog 2013; (9):2 e1003148-e1003148
    ID:
    PN135331
    • Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes

    作者
    Telford WG
    杂志
    Others (2004) 61A:9-17
    ID:
    PN129979
    作者
    Perfetto SP, Chattopadhyay PK, Roederer M
    杂志
    Nat Rev Immunol (2004) 4:648-655
    ID:
    PN56854
    作者
    Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C
    杂志
    Nature (321):431-434
    ID:
    PN130161
    作者
    van den Elsen P, Shepley BA, Borst J, Coligan JE, Markham AF, Orkin S, Terhorst C
    杂志
    Nature (312):413-418
    ID:
    PN130220

    详细说明

    常用规格

    Reactivity:

    Human

    Validated Application:

    Flow Cytometry

    Host:

    Mouse

    SubClass (Isotype):

    IgG2a

    Clonality:

    Monoclonal

    Clone ID:

    S4.1

    Target Gene:

    CD3

    Gene ID:

    915

    Label or Dye:

    Qdot® 655

    Excitation⁄Emission (nm):

    405(488)⁄655

    Number of Reactions:

    25 Tests

    Product Size:

    25 µL

    Shipping Condition:

    Wet Ice

    Regulatory Statement:

    For Research Use Only. Not for use in diagnostic procedures.

    内容和储存

    This product is supplied as 25uL of a 1 µM solution (in 50 mM borate, 1 M betaine, pH 8.3 that contains 0.05% sodium azide). Store at 2–8°C. Do not freeze.

    荧光光谱

    Qdot 655

    Fluorescence Ex/Em spectra of Qdot® 655 in pH 7.2 buffer.

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