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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Invalid
- 英文名:
FERRIC FERROCYANIDE
- 库存:
25g
- 供应商:
西宝生物
- 规格:
BIOCHEMICALS
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
and oxidation reactions require transfer of an electron , which is facilitated by electron acceptors of the proper redox potential (Cotson and Holt, 1958). The ferric and ferrous ions included in most X-gal reaction buffers provide this function (Lojda, 1970
and ferrocyanide can form a blue precipitate (Prussian blue) upon reaction with free ferric ion. Do not use metal forceps to manipulate the tissue while it is in the X-gal detection buffer. iv. All tissues have endogenous, lysozomal ß-gal. Its pH optimum
Celestine Blue 2.5g Ferric Ammonium Sulphate (Iron Alum) 25g Glycerin 70ml Distilled water 500ml Dissolve ferric ammonium sulphate in cold distilled water. Add celestine blue and boil for a few mins. Cool, filter and add glycerin
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