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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
6个月
- 英文名：
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]
- 库存：
10
- 供应商：
天津百萤生物科技有限公司
- 保存条件：
建议低温冷冻保存
有许多方法标记寡聚核苷酸，使其带有荧光。依据其光谱需求，标记的选择多元化。5-荧光素-CE-亚磷酰胺，来源于6-羧基荧光素单一异构体。5’-六氯荧光素氨基磷酸酯(HEX)和5‘-四氯荧光素氨基磷酸酯都可以在5-位高效的用于寡聚核苷酸标记。
用荧光素标记多聚糖3~-位可以获得 11’-Fluorescein CPGs，使用任何这类产品的荧光素标记的寡核苷酸都可以在氢氧化铵标准裂解条件下去保护。
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文献和实验Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
This protocol is for four time-points in 12-well plates. Scale up or down as needed. Day 1: Plate cells: Split one or more 10-cm plate(s) with 80-100% confluent HeLa Tet-off cells to a final volume of 10-11 ml. Make sure
Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression
of transgenic lines have been produced akin to those found in mouse and zebrafish. Here, I describe the design and characterization of transgenic lines in Xenopus constituting the Tet-On binary transgene expression system comprising two components: (1) rtTA
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
, the tet-system has proven to facilitate such strict control by tightly regulating gene expression, thereby assuring high expression levels in almost all organs and tissues. Yet, most of these results were obtained from the analysis of either selected cell
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