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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-15℃
- 保质期:
24个月
- 英文名:
MCA [7-Methoxycoumarin-4-acetic acid]
- 库存:
1
- 供应商:
西安百萤生物科技有限公司
- CAS号:
62935-72-2
- 规格:
1 g
MCA 7-甲氧基香豆素-4-乙酸基本资料
产品名称:MCA 7-甲氧基香豆素-4-乙酸 CAS 62935-72-2
英文名称:MCA [7-Methoxycoumarin-4-acetic acid] *CAS 62935-72-2*
产品货号:557
产品规格:1g
品 牌:AAT Bioquest
储存条件 :在零下15度以下保存, 避免光照
保质期:12个月
MCA 7-甲氧基香豆素-4-乙酸产品物理化学光谱特性
分子量:234.2
外观:灰白色固体
溶剂:DMSO
激发波长(nm):322
发射波长(nm):381
MCA 7-甲氧基香豆素-4-乙酸产品概述
AAT Bioquest生产的MCA (7-甲氧基香豆素-4-乙酸)用于分析蛋白酶活性的FRET肽底物。常与(DNP)基团配对。也用于荧光检测的HPLC衍生化。百萤生物是AAT Bioquest的中国代理商,为您提供优质的MCA 7-甲氧基香豆素-4-乙酸。
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cycles of 95° C for 1 min and 72° C (JXMA24) or 77° C (JMCA24) for 3 min. Final extension is at 72° C for 10 min. Transfer the PCR products to a clean microcentrifuge tube. To digest the single stranded amplified MCA products
Methylated CpG Island Amplification (MCA)
Methylated CpG island Amplification Protocol written by Minoru Toyota 2. Materials 2.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH
transfection. Maintain the cells for another 72 hto allow suffi cient time for HR mediated by CRISPR-Cas9 systemand the template. 3.5 Verifi cation and Quantifi cation of HR Effi ciency To verify the homologous recombination between the HR
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