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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
150 mM NaCl 5M stock 1.5 ml SDS 5 g Fill up to a total volume of 50 ml with H2 O (12) Sonicate again on ice (10 sec. pulse + 50
E. Z. N.A.TM X-press Plasmid Protocol
) into the Xpress spin column. 11. Centrifuge at maximum speed (13,000 x g) for 1 minute. 12. Discard the flow-through liquid and re-use the collection tube. 13. Place the empty X-press spin column back into the 2 ml collection tube. Centrifuge
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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