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文献和实验Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay
Identifying substrates of receptor and non-receptor protein tyrosine kinases (PTK), and how phosphorylation of these substrates affects signaling and cytoskeletal pathways, has been a key step in understanding the role of PTK in differentiation
phosphorylation, and downmodulate cellular protein tyrosine kinase activities (1 –6 ). These LMP2A functional studies have involved in vitro assays to determine the phosphorylation patterns of both LMP2A and cellular signal transducers before and after BCR
Immunoprecipitation is one of the most commonly used techniques to study protein-protein interaction in vivo. There are three major steps involved in an immunoprecipitation procedure: 1) lysis of the cells to make the antigen
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