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- 2026年01月14日
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- 文献和实验
- 技术资料
- 抗体名：
AFP Antibody (N-term)
- 抗体英文名：
AFP Antibody (N-term)
- 应用范围：
WB
- 适应物种：
H
- 克隆性：
多克隆
- 规格：
100UL
WBPrimary AccessionP02771Other AccessionNP_001125.1ReactivityHumanIsotypeMouse IgMClone Names143CT50.11.8Calculated MW68678 DaAFP Antibody (N-term) - Additional infoGene ID174Other Names
AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulinTarget/Specificity
This AFP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human AFP.Dilution
WB~~1:100~500Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Precautions
AFP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.AFP Antibody (N-term) - Protein InformationNameAFPSynonymsHPAFPFunction
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.Cellular Location
Secreted.Tissue Location
Plasma. Synthesized by the fetal liver and yolk sac
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文献和实验In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
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