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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
ProtOn™ Fluorescein Labeling Kit
- 宿主:
不限
- 抗原来源:
不限
- 库存:
大量
- 目录编号:
PLK-1201
- 供应商:
西宝生物
- 是否单克隆:
0
- 规格:
1 kit
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文献和实验Labeling of Oligonucleotides with Fluorescein
. They are amenable to several labeling techniques, both radioactive and nonradioactive; such methods include chemical modification during synthesis and the enzymatic incorporation of labeled nucleotides at the 5′ end or the 3′ end.
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
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