Figure 1. 比较SignaLOCK HRP ChemiWestern Kit (Imager), SuperSignal West Dura, and ECL Prime and their recommended blockers. Immunoblots were imaged for 1 minute after application of substrate. The SignaLOCK blot bands represent namogram to picogram detection of protein.
Figure 2. Comparison of femtogram sensitivity, with 20-minute exposure using SignaLOCK HRP ChemiWestern Kit (Imager), SuperSignal West Dura, and ECL Prime and their recommended blockers. Membranes were blocked overnight and protein-detected using a buildup of rabbit antilysozyme, followed by HRP-labeled goat anti-rabbit IgG (H+L).