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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
1226056-71-8
- 规格:
10 mM * 1 mL/1 mg/5 mg/10 mg/50 mg/100 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥880.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥363.0 |
| 规格: | 5 mg | 产品价格: | ¥800.0 |
| 规格: | 10 mg | 产品价格: | ¥1400.0 |
| 规格: | 50 mg | 产品价格: | ¥4900.0 |
| 规格: | 100 mg | 产品价格: | ¥8000.0 |
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Thiazovivin
CAS No. : 1226056-71-8
MCE 国际站:Thiazovivin
产品活性:Thiazovivin 是一种有效的 ROCK 抑制剂,对人胚胎干细胞具有保护作用。Thiazovivin 能提高了 iPSC 生成效率。
研究领域:Cell Cycle/DNA Damage | Stem Cell/Wnt | Cytoskeleton | TGF-beta/Smad
作用靶点:ROCK
In Vitro: Thiazovivin is a ROCK inhibitor. Thiazovivin (2 μM) inhibits ROCK activity and protects human embryonic stem cells (hESCs). Thiazovivin significantly increases the survival of hESCs after dissociation while maintaining pluripotency. Thiazovivin enhances cell-ECM adhesion-mediated integrin signaling. Thiazovivin also stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions. Thiazovivin increases cellular attachment of embryo-derived stem-like cells (eSLCs) of cattle and formation of primary colonies on the feeder layer. Thiazovivin reinforces putative colony outgrowth and supports the expansion of eSLC cultures during the subculture for passaging. Furthermore, Thiazovivin causes greater expression of ectodermal lineage-specific genes in eSLCs of cattle.
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文献和实验- - - - Thiazovivin 2.5mM - - - - Collagenase II - 0.4% - - - Hyaluronic acid - 0.1% - - - Trypsin-EDTA - 0.03% - - - Accutase 1× - B27 - - - 2% - N2
hPSC 分化缓冲液 DXF● hPSC-ECM DXF,20x 浓缩● Dulbecco PBS,无 Ca++/Mg++冻存 hPSC 细胞复苏或单细胞传代培养的需求:● Y-27632 (PK-CA577-1596-1) 或二者择其一● Thiazovivin (PK-CA577-1681-1) Ⅱ. 培养指导 该培养方案描述了 PromoCell 的 hPSC 生长培养基 DXF 转换目前培养的步骤。确保细胞培养状态良好。多潜能性最佳结果,应该≥90%。 1. 培养瓶包被
在了可生成结缔组织的成纤维细胞生成的自然过程研究上。这种自然发生过程称为MET(间质—上皮细胞转化),会使成纤维细胞更接近于干细胞样状态。研究小组测试了大量类药分子,寻找抑制TGFb(转化生长因子β)和MEK(裂原活化蛋白激酶)的路径。TGFb和MEK都参与MET过程。经过研究,他们发现两种化学物质——ALK5抑制剂SB43142和MEK抑制剂PD0325901——的组合,在促进成纤维细胞转化成干细胞方面的效用最高,比传统方法的效率高100倍。随后,研究人员又锁定了一种名为Thiazovivin的新型
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