- ¥578
- life
- 12800-017
- 2026年01月01日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
2
- 供应商：
研卉生物
- 英文名：
DMEM, Powder, High Glucose, Pyruvate (Gibco®)
- 规格：
10*1L
DMEM, Powder, High Glucose, Pyruvate (Gibco®)
货号：12800-017
描述
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With Without
• High Glucose • HEPES
• L-glutamine • Sodium Bicarbonate
• Phenol Red
• Sodium Pyruvate
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
详细说明
常用规格
| Glutamine: | L-Glutamine |
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| Glucose: | High Glucose |
| Serum Supplementation: | Standard Serum Supplementation |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Product Size: | 10 x 1 L |
| Sodium Bicarbonate Buffer: | No Sodium Bicarbonate |
| Shipping Condition: | Room Temperature |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验和脂质体溶液轻柔混匀。室温孵育20min 4、用胰酶消化并记数293T细胞。用含血清的培养基重悬细胞。 5、在六孔板中每孔，加入1ml含血清的生长培养基，再加入DNA-脂质体复合物。 6、将1ml重悬的293T细胞（1×106个细胞/ml）加入到平板中。37℃CO2孵箱中孵育过夜。 7、移除含有DNA-脂质体复合物的培养基移除，代之以DMEM（含丙酮酸钠和非必须氨基酸）。 7、转染后48-72h收获含病毒的上清。3000 rpm 离心20min，去除沉淀
瓶。你的培养基最好换换，可以多查文献啊，一般是高糖的DMEM+5％FBS+10%马血清（我的经验是Gibco的马血清贴壁最好）；马血清的功能是抑制PC12细胞形态的变化，有利于贴壁生长，不会抑制你用NGF诱导的PC12分化的；胎牛血清的作用是与细胞的增殖有关。PC12换液不要太勤！3到4天换液都行，并且最好保留一部分（如三分之一）旧培养基，有利于细胞的贴壁与增殖！！你传代时用胰酶消化吗？？有人说用0.025％的胰酶消化30秒就足够了，你可以试试，我没有用胰酶也行，为了让细胞不起团，我用了0.02％
时间不宜超过 2min。 25、将细胞悬液转移至 15ml 离心管中，加入 10ml 预冷的 DMEM/F12 培养基（含 10% FBS）。4℃ 条件下 220g 离心 5min，取细胞沉淀。 26、将所得细胞重复 8-12 步骤，完成 OC 类器官复苏。 图 2. OC 类器官的不同形态表型。即致密（左），囊性（中）和低内聚（右）型 OC 类器官。比例尺 200 μm【4】。 卵巢癌类器官样本来源不局限于活检样本或手术样本，腹水和胸腔积液中的癌细胞也同样可以用于构建 OC 类器官。培养
技术资料暂无技术资料 索取技术资料
