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- 12800-017
- 2026年01月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
2
- 供应商：
研卉生物
- 英文名：
DMEM, Powder, High Glucose, Pyruvate (Gibco®)
- 规格：
10*1L
DMEM, Powder, High Glucose, Pyruvate (Gibco®)
货号：12800-017
描述
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With Without
• High Glucose • HEPES
• L-glutamine • Sodium Bicarbonate
• Phenol Red
• Sodium Pyruvate
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
详细说明
常用规格
| Glutamine: | L-Glutamine |
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| Glucose: | High Glucose |
| Serum Supplementation: | Standard Serum Supplementation |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Product Size: | 10 x 1 L |
| Sodium Bicarbonate Buffer: | No Sodium Bicarbonate |
| Shipping Condition: | Room Temperature |
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文献和实验瓶。你的培养基最好换换，可以多查文献啊，一般是高糖的DMEM+5％FBS+10%马血清（我的经验是Gibco的马血清贴壁最好）；马血清的功能是抑制PC12细胞形态的变化，有利于贴壁生长，不会抑制你用NGF诱导的PC12分化的；胎牛血清的作用是与细胞的增殖有关。PC12换液不要太勤！3到4天换液都行，并且最好保留一部分（如三分之一）旧培养基，有利于细胞的贴壁与增殖！！你传代时用胰酶消化吗？？有人说用0.025％的胰酶消化30秒就足够了，你可以试试，我没有用胰酶也行，为了让细胞不起团，我用了0.02％
时间不宜超过 2min。 25、将细胞悬液转移至 15ml 离心管中，加入 10ml 预冷的 DMEM/F12 培养基（含 10% FBS）。4℃ 条件下 220g 离心 5min，取细胞沉淀。 26、将所得细胞重复 8-12 步骤，完成 OC 类器官复苏。 图 2. OC 类器官的不同形态表型。即致密（左），囊性（中）和低内聚（右）型 OC 类器官。比例尺 200 μm【4】。 卵巢癌类器官样本来源不局限于活检样本或手术样本，腹水和胸腔积液中的癌细胞也同样可以用于构建 OC 类器官。培养
NaHCO3 浓度的 Earle's 平衡盐溶液，并且由培养箱维持 5～10% CO2。通常，1.2～2.2g/L NaHCO3 配合 5% CO2，3.7 g/L NaHCO3 配合 10% CO2。精确的计量因培养基配方而不同。除了 Leibovitz's L-15 (ATCC No.30-2008)，ATCC 所有培养基都要匹配 5% CO2。绝大多数 ATCC 培养基含 1.5g/L NaHCO3 以及额外的丙酮酸钠。而 Gibco Hyclone 很多品牌的培养基里都含有 3.7 g/L
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