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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
most of it will pellet along with the bacterial cell wall. However the relative purity of the plasmid DNA will drop if the plasmid is large or at a relatively low copy number in the prep. The combination of a kit prep with cesium banding eliminates this problem
Endothelial Cell Systems - Instructions For Use
= 30 x 10,000 = 300,000 cells/ml. c.Determine the total number of cells in the total suspension volume. 1)Determine the total volume of the cell suspension. 2)Multiply the volume of the cell suspension by the "cells/ml" value calculated
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
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