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文献和实验Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
1.1. Can the NCIS Kit be used on adults? The NCIS kit is specifically packaged for five separate tissue dissociations, each containing up to twelve neonatal rat hearts. The NCIS protocol is available online at www.worthington-biochem.com
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
in transglycosylation reaction. In the assay, UDP was coupled to the conversion of phosphoenolpyruvate to pyruvate using pyruvate kinase. Using a commercial pyruvate assay kit, the pyruvate was converted to a red terminal product, which could be photometrically measured
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