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文献和实验. Mix by slowly stirring contents. Incubate overnight at 16°C. After ligation, carry out drop-dialysis of sample against approximately 25 ml 0.5 X TE, 1 X PA for 2 hours at room temperature in a 100 mm petri dish. 1 X PA is a mixture of spermine
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
. Incubate the samples for 30 min at 37°C. 22. Extract once with an equal volume of Phenol:Chloroform. Mix well. 23. Centrifuge at 16,000 X g at room temperature for 10 min. 24. Collect the upper, aqueous phase. 25. Add 7.5 M Ammonium Acetate, pH
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