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文献和实验Method for Single-Cell Electroporation
Inroduction Single-cell electroporation (SCE) is a technique we have developed to deliver genes into individual cells within intact tissues, although it may also be applicable to cells in disperse
From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism
, or Cell Lines Basic Protocol 1: Cuvette‐Based Spectrofluorometry to Assess Second‐Messenger Signaling in Living Cells Support Protocol 1: Enhanced Calcium Loading Techniques with Fura‐2 and Fluo‐4
Preparation of cytospin from single cell suspension.
cytocentrifuge (be careful, this is not biohazard proof!!) Prepare the slides mounted with the paper pad and the cuvette in the metal holder . Load up to 200 µl of this suspension in each cuvette. Spin at 800 rpm for 3 min. Extract
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