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文献和实验Lipoprotein Isolation - First week
condensation in the centrifuge. 3. Using a sharp single edged razor carefully cut open centrifuge tube. 4. Set up the BioRad fraction collector. Fractionate content into 20 two milliliter fractions. 5. Determine density using
Lipoprotein Isolation - First week
carefully cut open centrifuge tube. 4. Set up the BioRad fraction collector. Fractionate content into 20 two milliliter fractions. 5. Determine density using the refractometer . 6. Also determine UV absorbance at 280 nm. Remember to use
DNA Purification-Modified CTAB Procedure
wash buffer", and 0.1% b-mercaptoethanol and bring to room temperature. Move to orange cap tubes. 7.add 1/5 volume of 5% sarkosyl, set for 15 minutes at room temperature. 8.add 1/7 volume 5M NaCl; mix gently by inversion. 9.add 1/10
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