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文献和实验High performance liquid chromatography (HPLC) is the gold standard methodology for the HbA1c test. By manipulating the flow rate of the liquid (i.e. mobile phase) and the temperature of the HPLC column (i.e. stationary phase), it is possible
Dideoxyfingerprinting for Mutation Detection
Screening for mutations of the thousands of the sequence products provided by human genome analysis has proven to be a daunting task. The gold standard for identifying sequence alterations is direct sequencing. However, this method is labor
to MS/MS , Step 4) to which is also attached a gold wire to supply a spray voltage of ~1800 V, a microcapillary split line to provide an effective flow rate of 100-300 nl/minute, and a solvent line from the HPLC. 11. Equilibrate the column with 100
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