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文献和实验(Misonix XL 2020) for 3x6 sec, with a 6 sec intermission between each burst. See “Notes” below on sonication. 7. Examine the sonicated nuclei under a phase contrast microscope. The majority of the nuclei should have been lysed, while the nucleoli
METHOD Grow a 1-liter culture of the appropriate strain of E. coli (e.g., Y1090hsdR, XL1-Blue, or DH1) to stationary phase. Recover the bacteria by centrifugation at 4000g (5000 rpm
-----------------------------------------------------------------------------hi ,allI want to ask that the Tm's of AP1 and AP2 are 59¡ãC and 71¡ãC,which is written in the USER MANUAL,but I get 51.1¡ãC and 57.9¡ãC in the software of PRIMER PREMIER 5.0. If I use different softwares I will get different
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