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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
26.30 32.00 39.25 2 5.99 9.21 13.81 17 27.59 33.14 40.79 3 7.81 11.34
Labeling oligonucleotides with 32PATP
Mix the following in an eppendorf tube: 1. 0.5 microgram oligonucleotide dissolved in H2 O. 2. 3 microliters 10x kinase buffer. 3. 2 microliters 32 P ATP from ICN (>5000 ci/mmole). 4. H2 O so that the final volume is 30
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