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文献和实验Q: Does the PCR reaction volume (negatively) influence the outcome? A: No, especially since the introduction of the small, thin walled, 0.2 ml plastic vials fitting the 96 well metal blocks of the thermocyclers. A number of observations are worth
Quantitation of Protein:Micro-Kjeldahl Method
【 Purpose 】 Study the principle and the operational techniques of micro-Kjeldahl method. 【 Principle 】 The Kjeldahl method measures the nitrogen content of a compound and may be used to determine
Isolation of micro-RNA (miRNA)
succinic acid) β-Mercaptoethanol (β-ME) (14.4 M) Glycogen (2 mg/ml) Diethylpyrocarbonate (DEPC) DEPC-treated water 75% (v/v) ethanol (prepared using DEPC-treated water) Dounce, micro-Dounce, or mechanical
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