- ￥100 - 9999
- Bechman COULTER
- A36398
- 2025年07月08日
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文献和实验Single tube confirmation PCR protocol
concentration of ~10 µM). We use 5 µl of each primer in 50 µl PCR reactions (~ 1 µM final primer concentration). - Label 20 thin-wall PCR tubes (5 for each isolate) and add the following primer pairs: A-B, A-kanB, C-D, kanC-D, and A-D. Tubes 1-5
Single tube confirmation PCR protocol
as template. 3. PCR reactions - The lyophilized confirmation primers (~5-10 nmoles of each oligonuleotide) should be resuspended in 750 µl of TE (final concentration of ~10 µM). We use 5 µl of each primer in 50 µl PCR reactions (~ 1 µM final primer
from one tube to the other. 2 . The following solutions need to be prepared: Tag 5U/µl Primers TH132/TH133 (25 µM ) Outer
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