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文献和实验E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
Note: The following protocol is based on using OBI’s vacuum manifold (Product No. VAC-03). 1. Set up vacuum manifold by following manufacturer’s instructions. 2. Place waste collection tray inside the vacuum manifold, then place
E-Z 96® M13 Isolation Vacuum Manifold Protocol
1/5 volume of MPG Buffer (200μl MPG per 1 mL culture) to the M13 supernatant and mix by vortexing. Incubate at room temperature for 15 minutes. 5. Assemble the vacuum manifold by following the manufacturer’s instructions. If using Vac-03 vacuum manifold
E-Z 96 X-press Plasmid Vacuum Protocol
with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate
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