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文献和实验Lowry � Protein Determination (From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Waterborg and Harry R. Matthews) (Lowry, O. H., Rosebrough, N. J., Farr, A. L
Bicinchoninic Acid (BCA) Protein Assay (Smith)
below. Principle BCA serves the purpose of the Folin reagent in the Lowry assay,namely to react with complexes between copper ions and peptide bonds to produce a purple end product.The advantage of BCA is that the reagent is fairly stable under alkaline conditions
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
, contaminating the sterile media. 1. After sterilization and cooldown, increase the airflow until the vessel pressure reaches ~10 psi. 2. Shut down the air service valve at the copper pipe on the wall of the fermentor room. This activates the exhaust
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