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文献和实验Chromatin Immunoprecipitation (ChIP)
and immunoprecipitation (O’Neill and Turner 2003). Many variations on the basic protocol provided here have been used and can be found in the literature (Kuo and Allis 1999; Orlando 2000; Johnson and Bresnick 2002; O’Neill and Turner 2003; Ren and Dynlacht 2004
Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法一
.For 10ml Corning tubes,use scale 7 Spin 4℃,5min Transfer supernatant to a new tube. To each lysate,add 60μl 50% Glutathione-Sepharose 4B Pepette 400μl Sepharose stock (75%) Spin 1000rpm 5min,discard supernantant Wash 3x300μl NETN Resuspend in 300μl
RLCS (Restriction Landmark cDNA Scanning) Protocol
in liquid nitrogen. Usually mortor and pestle are suited. A mechanical cruasher, e.g. Multibeads Shocker MB301 (Yasui Kikai Corp.), is also applicable. Transfer the frozen powder to a 50-ml tube. The powder can be stored at -80o C. Morning
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